The original photograph of the woman in the viral "Disaster Girl" meme was sold for $473,000 (£341,000).
Zo Roth, now 21, became an internet sensation at the age of four when she was photographed standing in front of a burning building with a devilish grin on her face.
The image has become a meme for anyone attempting to indicate a tragic scene or nefarious act.
Ms. Roth auctioned it off as a non-fungible token (NFT) - a digital ownership certificate - on Thursday.
When the image was posted online after winning a photography prize in 2008, it quickly went viral.
Ms. Roth, who is now a university student, sold the original copy of her meme as an NFT to a collector named @3FMusic for 180 Ethereum, a type of cryptocurrency.
An NFT is a one-of-a-kind digital token that is encrypted with an artist's signature and is permanently attached to the piece. It enables the sale of original versions of popular online content, such as viral memes and tweets, as if they were physical works of art.
The NFT is labeled with a code that will allow the Roths, who have stated that they will split the profits, to retain the copyright and receive 10% of future sales profits.